Virtual Microscopy
Microscopy Primer
License Info
Image Use
Custom Photos
Partners
Site Info
Contact Us
Publications
Home

Visit Science,
Optics, & You


The Galleries:

Photo Gallery
Silicon Zoo
Pharmaceuticals
Chip Shots
Phytochemicals
DNA Gallery
Microscapes
Vitamins
Amino Acids
Birthstones
Religion Collection
Pesticides
Beershots
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery
 

Fascinating Photos with
a Simple Microscope

Michael W. Davidson
National High Magnetic Field Laboratory (NHMFL)
and Center for Materials Research and Tech
The Florida State University
Tallahassee, USA

Figure 1. Nebraska
"Nebraska" is a triple exposure of recrystallized ascorbic acid (the wheatlike plants in the foreground) stretched polyethylene (the morning sky), and the field-diaphragm image defocused and photographed through a yellow filter (the yellow sun).

Photography through the microscope, or more commonly, photomicrography, has long been a useful tool for scientists. However, anyone who has access to a simple light microscope can produce highly color-saturated photographs, which display an exciting blend of art and science. Simple light microscopes with high-quality optics can be purchased for as little as $250, and adapters for most cameras are available for $25 to $75 (see the accompanying list of microscope manufacturers and distributors). Photographers who have access to state and government-surplus facilities may often find rather expensive surplus microscopes available at reasonable prices. Expensive high-powered microscope objectives (lenses) have a very narrow depth of field and are not really useful; therefore, a lower-cost 5x or 10x objective, which usually comes, as standard equipment on most microscopes, is all that is needed. All of the photomicrographs in this article were taken using a Nikon 4x or 10x objective. You can add polarizers to your microscope for just a few dollars, and the use of polarized light allows you to get beautiful photomicrographs of crystals made from common household chemicals.

Figure 2. (above left) Nutrasweet
A single exposure of aspartame recrystallized from the melt produced these unusual forms. This amino-acid derivative has been found to prossess great sweetening power, and is the active ingredient in Nutrasweet®.

Figure 3. (above right) Vitamin B1
A single exposure of thiamine (vitamin B1) crystallized from slowly evaporating solution in ethyl alcohol produced this "bubbling" image.

GETTING STARTED

The first polarizer is inserted at the base of the substage condenser (shown in the microscope diagram on page 5) and can be held in place with tape. If the microscope has a built-in light source, the polarizer can be placed over the field lens. The second polarizer, commonly termed the analyzer, is placed inside the body of the microscope between the main-body tube and the eyepiece tube. There is usually a lens mount at the top of the body tube, and the analyzer can be placed directly on this mount. You will need a polarizer that is approximately 1-3cm in diameter because of the restricted area within the main body tube. This can be obtained by cutting a piece of polarized sheet plastic or buying a small polarizer from a science-supply distributor.

Figure 4. AZT
A single exposure of sulfur recrystallized from the melt produced this pattern. This pattern is a single exposure of 3'-azido-thymidine (AZT) recrystallized from the melt. This drug is currently the most clinically effective treatment for HIV-infected AIDS patients.

Next, rotate the polarizer until the viewfield becomes very dark. At this point, the polarization direction is perpendicular between the polarizer and the analyzer, and you have what is termed crossed polarizers. When purchasing polarizers, remember to select polarizers that are very close to a neutral gray in color, such as the threaded polarizers made for the front of a camera lens. Avoid polarizing materials that are green or amber in color.

Figure 5. DNA Flower
This leaf is a single exposure of a liquid crystalline solution of DNA. As the solution slowly evaporates, the DNA concentration increases and the solution enters liquid crystalline states. This photomicrograph approximates the state of DNA found in nature.

It is very important to ensure that your microscope is aligned to produce even illumination across the viewfield. Information in microscope alignment is available in owner's manuals or in textbooks dealing with microscopy. An exceptionally good book, Photography Through the Microscope, is available from Kodak (Publication P-2), and can be purchased in many camera shops.

Figure 6. Mexico Beach, Florida
"Mexico Beach, Florida" is four exposures on a single frame of film: recrystallized ascorbic acid (the sandy, rocky foreground), stretched polyethylene with a blue filter (the ocean and morning sky), and the field diaphragm image defocused (two exposures-the rising sun, and its reflection photographed through a comb "diffraction grating").

Most crystals are anisotropic and birefringment, which means that they will refract plane-polarized light emitted from the polarizer, and will bend it until it is visible through the analyzer when the polarizers are crossed.

To prepare crystals for examination in the microscope, you simply deposit a few granules of the crystal onto a microscope slide or a thin piece of 2 x 2-inch glass. Next, carefully place a microscope coverslip, easily obtained from a hobby shop or science-supply shop, over the crystals and heat slowly with a match or cigarette lighter until the crystals melt completely (a bunsen burner or alcohol lamp will do a very good job, if available). When melted, the crystals will flow underneath the coverslip and fill the entire volume between the coverslip and fill the entire volume between the coverslip and the microscope slide. Allow the slide to cool, and place it in a safe place for a few days to permit the melted chemical to slowly recrystallize. Very thick crystal samples will usually not transmit much light, and are not as colorful as thinner preparations. If you don't like the crystal formations after recrystallization, re-melt the sample and allow it to recrystallize a second time.

Figure 7. Aspirin
How do you see relief? This kaleidoscopic image is a single single exposure of pain-reliever aspirin recrystallized from the melt.

Another effective method of preparing crystals is to dissolve the chemical in a suitable solvent, like water, rubbing alcohol, or mineral spirits. This method is especially useful for chemicals in the salt family, like table salt, Epsom salts, Alka-Seltzer, and baking soda. Salts will usually decompose when heated, leaving a tarlike mess. However, when dissolved in water or alcohol, these salts will slowly recrystallize as the solvent evaporates to form colorful crystalline patterns. Many chemicals can form different types of crystals, depending on whether they are melt-recrystallized or recrystallized from solution. Some chemicals recrystallize almost immediately, while some take hours, days, or even weeks. The chemicals listed in the accompanying table are easily obtained, and will recrystallize in a reasonable amount of time to yield beautiful photomicrographs. Several examples of photomicrographs of household chemicals are illustrated.

Figure 8. Feathers
"Feathers" was produced by a single exposure of the sugar arabinose recrystallized from the melt.

CHOICE OF FILM

The majority of microscopes use a tungsten-halide bulb as a light source. These bulbs emit a very bright light with a wavelength spectrum centered in the 3200 K color-temperature range. There are a wide variety of films available that reproduce this color balance correctly.

Figure 9. Ascorbic Acid
"Bizarre Windows" is a single exposure of absorbic acid (vitamin C) recrystallized from molten chemical.

Kodak's Ektachrome 50 and 160 are good examples of transparency films that are tungsten-balanced; however, I prefer the good contrast and color saturation provided by Fujichrome 64T. Photomicrographs are notoriously low in contrast, so I usually underexpose one or two f-stops and push E-6 processing one f-stop. This increases the contrast and color saturation, without a significant loss in density.

Figure 10. Stand
"The Stand" consists of four exposures: recrystallized ascorbic acid (the plantlike structures found in the foreground), a blue filter (the sky), tiny ascorbic-acid crystallites (the stars), and the field diaphragm image defocused with a ballpoint pen inserted into the light path to resemble a new moon.

For Kodachrome lovers, Kodak makes a fine-grained ISO 40-speed, 3400 K-balanced Kodachrome, which can be corrected, for a tungsten-halide light source by the addition of a No. 82A filter between the light source and the first polarizer. If you are in a hurry, Polaroid's High Contrast Polachrome HCP (ISO 40) will produce very good results. With any film, it's wise to bracket the first roll to get a handle on exposure times.

Figure 11. Lover's Leap
"Lover's Leap" is another quadruple exposure: recrystallized sulfur (the canyon), a blue filter (the sky), tiny ascorbic acid crystallites (the stars), and the field diaphragm defocused with a yellow filter (the sun or moon).

Common chemicals suitable for recrystallization.

CHEMICAL COMMENTS

Alka-Seltzer - Best crystals from aqueous solution.

Aspartame (Nutra-sweet) - Good crystals from melt-recrystallization or aqueous solution.

Aspirin (acetylsalicylic acid) - Equally good crystals from melt-recrystallization or aqueous or ethanol solutions.

Acetaminophen (Tylenol) - Equally good crystals from melt-recrystallization or aqueous solution.

Epsom salts - Recrystallize from aqueous solution only.

Glucose and sucrose - Crystals quickly obtained (common sugars) by evaporation of solution in water. Very beautiful crystals after melt-crystallization.

Kodak Dektol paper developer - Best crystals after evaporation of working stock solution.

Kodak D-76 film developer - Best crystals after evaporation of working stock solution.

Figure 12. Sulfur
A single exposure of sulfur recrystallized from the melt produced this pattern.

Microscope and accessory manufactures and distributors

OLYMPUS CORPORATION
4 Nevada Drive
Lake Success, NY 11042
Telephone: 516-488-3880
CAROLINA BIOLOGICAL
SUPPLY COMPANY
2700 York Road
Burlington, NC
Telephone: 800-334-5551
E. LEITZ, INC.
24 Link Drive
Rockleigh, NJ 07647
Telephone: 201-767-1100
CARL ZEISS, INC.
One Ziess Drive
Thornwood, NY 10594
Telephone: 914-747-1800
NIKON INC. INSTRUMENT GROUP
623 Stewart Ave.
Garden City, NY 11530
Telephone: 516-222-0200
AO SCIENTIFIC INSTRUMENTS
P.O. Box 123
Buffalo, NY 14240
FISHER SCIENTIFIC
50 Fadem Road
Springfield, NJ 07081
Telephone: 201-379-1400
EXCEL TECHNOLOGIES
90 Phoenix Avenue
Enfield, CT 06082
EDMUND SCIENTIFIC CO.
101 E. Gloucester Pike
Barrington, NJ 08007
Telephone: 609-573-6250
McCRONE ACCESSORIES AND COMPONENTS
850 Pasquinelli Dr.
Westmont, IL 60559
Telephone: 312-887-7100

Michael W. Davidson is a Research Associate at the Institute or Molecular Biophysics at Florida State University.

Questions or comments? Send us an email.
© 1995-2013 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, software, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last Modification Friday, Aug 01, 2003 at 11:43 AM
Access Count Since April 5, 1998: 279437
Microscopes provided exclusively by: