Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Photomicrography
Microscopy Museum
Virtual Microscopy
Fluorescence
Web Resources
License Info
Image Use
Custom Photos
Partners
Site Info
Contact Us
Publications
Home

The Galleries:

Photo Gallery
Silicon Zoo
Pharmaceuticals
Chip Shots
Phytochemicals
DNA Gallery
Microscapes
Vitamins
Amino Acids
Birthstones
Religion Collection
Pesticides
BeerShots
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Interactive Java Tutorials

Hoffman Modulation Contrast

This interactive tutorial explores how rotation of the substage polarizing filter affects image contrast in Hoffman modulation contrast microscopy. Instructions for operation of the tutorial appear below the applet window.

Interactive Java Tutorial
ATTENTION
Our servers have detected that your web browser does not have the Java Virtual Machine installed or it is not functioning properly. Please install this software in order to view our interactive Java tutorials. You may download the necessary software by clicking on the "Get It Now" button below.

 

The tutorial initializes with a specimen randomly selected from the pull-down Choose a Specimen menu. Positions of the three sliders are also randomized when the tutorial is first launched or when a new specimen is selected. Use the Polarizer Rotation slider to simulate rotation of the substage polarizer to increase or decrease specimen contrast. The Brightness slider simulates microscope lamp illumination intensity and may be used to control specimen brightness. A Contrast slider is used to simulate varying degrees of polarizer extinction to adjust the amount of contrast observed in the specimen.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


BACK TO VIRTUAL MICROSCOPY

BACK HOFFMAN MODULATION CONTRAST

Questions or comments? Send us an email.
© 1998-2013 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Friday, Feb 28, 2014 at 04:10 PM
Access Count Since May 12, 2000: 63147
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: