Fluorescence Digital Image Gallery

Embryonic Swiss Mouse Fibroblast Cells (3T3)

The albino Swiss mouse embryonic fibroblast (3T3) cells presented in the digital image above were resident in an adherent culture stained for F-actin with Alexa Fluor 488 conjugated to phalloidin (green fluorescence), and for DNA with the bis-benzimidazole dye Hoechst 33258 (pseudocolored cyan). In addition, the culture was immunofluorescently labeled with Alexa Fluor 568 (pseudocolored red) and Alexa Fluor 750 (pseudocolored yellow) conjugated to goat secondary antibodies that target mouse anti-vimentin (intermediate filaments) and rabbit anti-giantin (targeting the Golgi complex) primary antibodies, respectively. The mitochondrial network was simultaneously visualized using MitoTracker Deep Red 633 (pseudocolored dark blue). Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a smaller image of the albino Swiss mice (3T3) cells.

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