Fluorescence Digital Image Gallery

Mink Uterus Endometrium Epithelial Cells (GMMe)

Mitochondria are often depicted as static, cylinder-shaped organelles, but time-lapse studies reveal that the important energy generators may change shape and move about the cytoplasm on an almost continuous basis. These activities often appear to involve the cytoskeletal microtubules, which influence the characteristic direction and dissemination of the mitochondria in various kinds of cells. The number of mitochondria present in a cell is related to the metabolic needs of that cell, and may range from a single large mitochondrion to thousands of the organelles. Their size generally ranges from 1 to 10 micrometers, making mitochondria large enough to be observed with a light microscope. The organelles were originally identified during the 1800s, and were commonly believed to transmit hereditary information until after the beginning of the twentieth century. Most of the modern understanding of the functional role of mitochondria did not develop until after a method for isolating the intact organelles was developed in 1948.

In what has become a favorite standard fluorescence staining protocol, the log phase monolayer adherent culture of GMMe cells presented above was labeled with MitoTracker Red CMXRos before fixing, and the cells were subsequently permeabilized and stained with Alexa Fluor conjugated to phalloidin, followed by DAPI, a popular DNA-binding counterstain. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a smaller image of the mink uterus endometrium epithelial (GMMe) cells.

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