Fluorescence Digital Image Gallery

Mouse Hemangioendothelioma Endothelial Cells (EOMA)

Deoxyribonucleic acid (DNA) is a chemical that was first isolated in the 1860s, although its significance in terms of genetics was not realized until the mid-twentieth century. The nucleic acid is found predominantly in the nuclei of eukaryotic cells, but certain other organelles, such as mitochondria and chloroplasts, also contain DNA. In prokaryotes, the nucleic acid is located throughout the entire cell. Oswald Theodore Avery was the first scientist to produce convincing evidence that DNA was responsible for the transmission of genetic information, but James Watson and Francis Crick were central to the discovery of how the nucleic acid carries out this function. Their determination that DNA exists as a double helix was a key step on the way to the modern understanding of genetic transmission.

The EOMA cell culture featured in the digital image above was labeled with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, targeting the intracellular mitochondrial network, cytoskeletal F-actin, and nuclear DNA, respectively. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a smaller image of the mouse hemangioendothelioma (EOMA) cells.

© 1995-2013 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, software, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Thursday, Oct 14, 2004 at 09:34 AM
Access Count Since July 16, 2004: 5152
Microscopes, fluorescence filters, and digital imaging equipment provided by:
Visit the Olympus Microscopy Resource Center website. Visit the Omega Optical website. Visit the QImaging website.