DiO & WB
The photomicrograph below illustrates The membrane-specific fluorochrome 3,3'-dipentyloxacarbocyanine iodide (DiO) used as a tracer with a green wide-pass WB fluorescence cube. Note that the excitation and emission spectra (green) have considerable overlap.
Objective: FL 20X
Photoeyepiece: PE 3.3X
Fluorescence Cube: WB
Exposure Mode: SFL 1
BACK TO FLUORESCENCE MICROSCOPY
Questions or comments? Send us an email.
© 1998-2013 by
Michael W. Davidson and The Florida State University.
All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
Last modification: Friday, Aug 01, 2003 at 11:43 AM
Access Count Since July 26, 1998: 9316
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: