Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Photomicrography
Microscopy Museum
Virtual Microscopy
Fluorescence
Web Resources
License Info
Image Use
Custom Photos
Partners
Site Info
Contact Us
Publications
Home

The Galleries:

Photo Gallery
Silicon Zoo
Pharmaceuticals
Chip Shots
Phytochemicals
DNA Gallery
Microscapes
Vitamins
Amino Acids
Birthstones
Religion Collection
Pesticides
BeerShots
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Differential Interference Contrast Image Gallery

Nucleic Acid Stains

The first synthetic dye, aniline purple, was developed in 1856 by English chemist William Perkin. Though other synthetic chemists soon replicated the feat, it was at least another twenty years before using dyes and stains became common use in most scientific laboratories.

In modern times, the process of staining cells to observe their features is widespread, and scientists have an array of stains, generally composed of heterocyclic organic dyes, to choose from. In order to make a suitable stain selection, the chemical and electronic properties of the dye molecules with respect to their interaction with cellular components must be considered. For instance, stains that have a net positive charge in aqueous or buffered solutions have an affinity for negatively charged biological structures and selectively bind to them. Hydrophilic stains, on the other hand, tend to stain hydrated biological bodies, such as the exterior of proteins and nucleic acids. Contrariwise, stains hydrophobic in nature concentrate on membranes, lipids, and the interior of proteins.

Ethidium bromide is a typical nucleic acid stain used by molecular biologists. Dark red and in crystal form when not in an aqueous solution, the fluorochrome exhibits a reddish-orange fluorescence that can be detected under both long and short ultraviolet wavelengths of light. Furthermore, ethidium bromide is a relatively large, flat basic molecule that somewhat resembles a DNA base pair and because of its structure can easily insert into a DNA strand. Thus, the substance is extremely useful for experimental purposes, though it can dangerous to handle and should be employed with caution.

BACK TO THE DIC IMAGE GALLERY

Questions or comments? Send us an email.
© 1998-2013 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Friday, Aug 01, 2003 at 11:43 AM
Access Count Since April 22, 2003: 18295
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: