Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Microscopy Museum
Virtual Microscopy
Web Resources
License Info
Image Use
Custom Photos
Site Info
Contact Us

The Galleries:

Photo Gallery
Silicon Zoo
Chip Shots
DNA Gallery
Amino Acids
Religion Collection
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Interactive Java & Flash Tutorials

We have constructed several interactive Java tutorials to help demonstrate many of the complex topics in photography through the microscope. Use the links below to navigate to specific tutorials of interest.

Color Temperature Conversion Calculator - Use this interactive Java tutorial to determine the correct color conversion filter for balancing the color temperature of a microscope light source.

Exposure and Color Balance - Explore how adjustments to exposure and color balance affect the quality of both ordinary photographs and photomicrographs.

Didymium Filters - These color filters act to enhance certain tones and hues in color photomicrography. Use this interactive tutorial to explore how didymium filters modify photomicrographs of specimens stained with eosin, fuchsin, and methylene blue.

Filter Classes - Filters are frequently utilized in optical microscopy, both for visualization of specimens and for photomicrography. This interactive Java tutorial explores the visible light spectral characteristics of the major types of filters used in microscopy.

Black Body Radiator Color Temperature - Discover how raising the temperature of a black body radiator affects its color temperature in the visible light region.

Color Temperature Nomograph - Use the color temperature nomograph interactive Java tutorial to calculate the filtration factor necessary to convert a light source from one color temperature to another.

Filters for Black & White Photomicrography - Experiment with this interactive Java tutorial to determine the appropriate starting point for contrast control in Black & White Photomicrography using Kodak Wratten filters.

Photomask Reticle Operation - Practice adjustment of the photomask reticle mounted in a focusing eyepiece using this interactive tutorial.

Illuminated Reticles - Microscopes equipped specifically for fluorescence illumination often have illuminated reticles in the eyepiece, which allow the microscopist to visualize faintly visible specimens superimposed against a very dark background. Use this interactive Java tutorial to practice examining fluorescence specimens with illuminated reticles by adjusting the focus and color of the reticle.

Exposure Metering - Discover how the automatic camera metering system works to measure light intensity in the field of view and calculate exposure times based on light intensity in different parts of the specimen.

Abbe Condenser Chromatic Aberrations - Abbe and aplanatic substage condensers are uncorrected for chromatic aberration, and even if they are properly adjusted using Köhler illumination, an undesirable color effect will be present in photomicrographs made using these condensers. This tutorial demonstrates color fringes that occur at the field diaphragm edges as a result of chromatic aberration in Abbe condensers.

Filter Control of Specimen Contrast in Black & White Photomicrography - To enhance contrast, color filters are added to the microscope light path to absorb stained specimen color, rendering it either a darker or lighter shade of gray. This tutorial explores the use of Kodak Wratten color filters for contrast control in black & white photomicrography when using stained specimens.

Exposure Calculation in Photomicrography - Use a known film exposure time measured from brackets under standardized conditions of magnification and numerical aperture to determine exposure for a new objective of different magnification and numerical aperture.

Exposure Calculation (With ISO Correction) in Photomicrography - Use a known film exposure time measured from brackets under standardized conditions of ISO, magnification, and numerical aperture to determine exposure for a new objective of different magnification and numerical aperture using a film with a new ISO value.

Exposure Characteristic Curves and Image Contrast - This interactive Java tutorial explores the relationship between the slope (often referred to as gamma) of characteristic curves exhibited by transparency and negative films and the amount of contrast produced by these films.

Charge-Coupled Device (CCD) and Digital Imaging Tutorials

Avalanche Diodes - An avalanche photodiode is a silicon-based semiconductor containing a pn junction consisting of a positively doped p region and a negatively doped n region sandwiching an area of neutral charge termed the depletion region. These diodes provide gain by the generation of electron-hole pairs from an energetic electron that creates an "avalanche" of electrons in the substrate.

Electron-Bombarded CCDs - Electron-bombarded charge-coupled devices (CCDs) are a relatively new development in which photons are detected by a photocathode in a manner similar to an image intensifier. The high-energy electrons that strike the CCD generate multiple charges resulting in a modest gain of a few hundred.

Binning - Discover how clock signals controlling a CCD can be used to combine integrated charge from adjacent pixels to improve signal-to-noise ratios and increase the readout frame rate.

CCD Blooming - Under conditions where a CCD is exposed to very high intensity illumination, it is possible to exhaust the storage capacity of the CCD wells, a condition known as blooming. When this occurs, excess charge will overflow into adjacent CCD photodiode wells resulting in a corrupted image near the blooming site. This tutorial explores the operation of a lateral overflow drain to prevent CCD blooming.

CCD Clocking Schemes - Charge transfer through CCD shift registers occurs after integration to relocate accumulated charge information to the sense amplifier, which is physically separated from the parallel pixel array. This tutorial explores several clocking schemes that are utilized to transfer charge from the collection gates to the output node.

Building A Charge-Coupled Device - Explore the steps utilized in the construction of a charge-coupled device (CCD) as a portion of an individual pixel gate is fabricated on a silicon wafer simultaneously with thousands or even millions of neighboring elements.

Building A Charge-Coupled Device (Version Number 2) - A second examination of the steps utilized in the construction of a charge-coupled device (CCD). Like the first version this tutorial examines the fabrication of a CCD as a portion of an individual pixel gate is fabricated on a silicon wafer simultaneously with thousands or even millions of neighboring elements.

CCD Operation - Explore the operation of a charge-coupled device (CCD) imaging semiconductor with this interactive Flash tutorial. Modern CCDs consist of a light-sensitive sandwich of insulating silicon dioxide positioned beneath an array of photodiodes and above an array of metal electrodes.

Electronic Shutters - Electronic shutters are employed in charge-coupled devices (CCDs) to control integration time (exposure) of the photodiode array and reduce smear when capturing moving objects in the microscope. Investigate the operation of an electronic shutter in controlling exposure using this interactive Java tutorial.

Frame-Transfer CCD Operation - Designed to operate fast and efficiently without a shutter or synchronized strobe, frame-transfer CCDs exhibit higher frame rates than full-frame designs. Explore image acquisition and transfer in frame-transfer charge-coupled devices with this interactive Java tutorial.

Full-Frame CCD Operation - Full-frame charge-coupled devices have the simplest architecture and are the easiest devices to build and operate. These devices feature high-density pixel arrays capable of producing digital images with the highest resolution currently available.

Interline CCD Operation - Interline charge-coupled device architecture is designed to compensate for many of the shortcomings of frame-transfer CCDs. These devices are composed of a hybrid structure incorporating a separate photodiode and a CCD storage region, protected with a mask structure, into each pixel element.

Interaction of Photons with Silicon - In a charge-coupled device (CCD) incident light must first pass through a silicon nitride passivation coating as well as several thin films of silicon dioxide and polysilicon gate structures before being absorbed into the silicon substrate. This interactive tutorial explores the interaction of photons with silicon as a function of wavelength.

Microlens Arrays - Microlens arrays (also referred to as microlenticular arrays or lenslet arrays) are used to increase the optical fill factor in CCDs, such as interline devices, that suffer from reduced aperture due to metal shielding. These tiny lens systems serve to focus and concentrate light onto the photodiode surface instead of allowing it to fall on non-photosensitive areas of the device, where it is lost from the imaging information collected by the CCD.

Photomultiplier Tubes - Photomultiplier tubes, useful for light detection of very weak signals, are photoemissive devices in which the absorption of a photon results in the emission of an electron. These detectors work by amplifying the electrons generated by a photocathode exposed to a photon flux. Explore electron amplification in photomultiplier tubes with this interactive Flash tutorial.

Channel Photomultipliers - Channel photomultipliers represent a new design that incorporates a unique detector having a semitransparent photocathode deposited onto the inner surface of the entrance window. Photoelectrons released by the photocathode enter a narrow and curved semiconductive channel. Each time an electron impacts the inner wall of the channel, multiple secondary electrons are emitted. These ejected photoelectrons have trajectories angled at the next bend in the channel wall (simulating a dynode chain), which in turn emits a larger quantity of electrons angled at the next bend in the channel. The effect occurs repeatedly, leading to an avalanche effect, with a gain exceeding 100 million.

Side-On Photomultiplier Tubes - In the side-on photomultiplier tube design, photons impact an internal photocathode and eject electrons from the front face. These ejected photoelectrons have trajectories angled at the first dynode, which in turn emits a larger quantity of electrons angled at the second dynode (and so on).

Proximity-Focused Image Intensifiers - Image intensifiers were developed for military use to enhance our night vision and are often referred to as wafer tubes or proximity-focused intensifiers. They have a flat photocathode separated by a small gap on the input side of a micro-channel plate (MCP) electron multiplier and a phosphorescent output screen on the reverse side of the MCP.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Kenneth R. Spring - Scientific Consultant, Lusby, Maryland, 20657.

Brian O. Flynn, John C. Long, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.



Questions or comments? Send us an email.
© 1998-2013 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Wednesday, Apr 13, 2005 at 08:03 AM
Access Count Since April 30, 2000: 38306
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: