Microscopy Primer
Light and Color
Microscope Basics
Special Techniques
Digital Imaging
Confocal Microscopy
Live-Cell Imaging
Photomicrography
Microscopy Museum
Virtual Microscopy
Fluorescence
Web Resources
License Info
Image Use
Custom Photos
Partners
Site Info
Contact Us
Publications
Home

The Galleries:

Photo Gallery
Silicon Zoo
Pharmaceuticals
Chip Shots
Phytochemicals
DNA Gallery
Microscapes
Vitamins
Amino Acids
Birthstones
Religion Collection
Pesticides
BeerShots
Cocktail Collection
Screen Savers
Win Wallpaper
Mac Wallpaper
Movie Gallery

Interactive Java Tutorials

Exposure Metering

Automatic cameras for photomicrography are equipped with a light-sensitive metering system that determines exposure time. These devices work by averaging light in the central 30 percent of the viewfield, and many are equipped with a spot metering option that allows light to be measured in a very small portion of the field.

Interactive Java Tutorial
ATTENTION
Our servers have detected that your web browser does not have the Java Virtual Machine installed or it is not functioning properly. Please install this software in order to view our interactive Java tutorials. You may download the necessary software by clicking on the "Get It Now" button below.

 

This tutorial simulates an automatic exposure metering system on a modern microscope. The rectangular area delineated in the viewfield represents the portion that will be captured onto 35 millimeter film. Crosshairs in the center are used for aligning focus between the focus finder and the film plane. Use the Metering radio buttons to select Average (over 10 percent of the viewfield) or Spot (1 percent of the viewfield) metering. When Average is selected, a yellow circle appears in the viewfield and a yellow dot approximating the size of the circle appears in the small window below. Use the sliders or mouse to move the circle around the viewfield to gauge light intensity at different locations on the specimen. As the metering circle is moved, the Exposure Time digital readout displays the calculated exposure time in seconds. Translate the circle around the viewfield to see how illumination varies at different parts of the field. Select the Spot radio button for a more accurate light measurement. The Reticle Color radio button allows the visitor to select a reticle display color from a palette that includes red, yellow, and black. Specimens can be selected from the Select a Specimen pull-down menu.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


BACK TO PHOTOMICROGRAPHY

Questions or comments? Send us an email.
© 1998-2013 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Wednesday, Mar 26, 2014 at 01:23 PM
Access Count Since April 19, 2000: 13891
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: