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Köhler Illumination: Interactive Java Tutorials

Aperture and Field Diaphragm
Adjustment in Reflected Light

This tutorial simulates the operation of a reflected light microscope and allows students to experiment with various settings for the sample focus, field diaphragm iris size and position, and the opening size of the aperture iris diaphragm.

Interactive Java Tutorial
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Each time the applet is reloaded, a new set of initial states is randomly selected. To operate the tutorial, use the sliders and radio buttons to configure the reflected light microscope for optimum performance. The slider on the far left controls specimen focus throughout the entire magnification range. The "Aperture Diaphragm" slider controls the size of the aperture iris diaphragm, which allows control over specimen contrast and image quality. Radio buttons at the bottom of the applet window control the selected magnification. The "Field Diaphragm Opening Size" slider controls the size of the field diaphragm iris, and the "Field Diaphragm Alignment" sliders control its position. The mouse cursor can also be used to navigate the field diaphragm around the viewfield from within the Diaphragm Alignment box. The Bertrand lens radio buttons toggle a "virtual" Bertrand lens into and out of the viewfield so the size of the aperture diaphragm can be monitored. Use the "Reset" button to reload a new configuration of initial values into the applet to practice aligning the microscope.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.


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