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Köhler Illumination: Interactive Java Tutorials
Substage Condenser
Numerical Aperture
This tutorial explores how the substage condenser diaphragm controls the numerical aperture of the light cone that illuminates the objective.
To operate the tutorial, simply move the slider up or down to change numerical aperture (this moves in a pre-determined set of steps). Note that changes in the condenser aperture diaphragm size result in modification of the size and angle of the illuminating light cone. This controls the numerical aperture of the microscope and regulates the quantity of light that enters the objective front lens.
Contributing Authors
Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.
Matthew J. Parry-Hill and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.
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© 1998-2008 by Michael W. Davidson, Mortimer Abramowitz, Olympus America Inc., and The Florida State University. All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
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