Organization of the Transition State

Organization of the Transition State

Structure of the transition region between cholesteric and high density liquid crystalline DNA phases has not yet been fully elucidated. The photomicrograph presented above illustrates this birefringent region, but yields few clues to the exact nature of the organization. The DNA concentration in this experiment is approximately 350 milligrams per milliliter, and the photomicrograph magnification is approximately 500x. Originally recorded on Fujichrome 64T transparency film using a Nikon Optiphot-Pol microscope with crossed polarized illumination, the image was digitized using a Nikon CoolScan transparency film scanner. Exposures were recorded about 3 f-steps under the recommended value given by an in-camera photomultiplier and were push-processed approximately 1.75 f-steps in the first E-6 developer.

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