Birefringence of the Transition Region

Birefringence of the Transition Region

Examination of the cholesteric to high density phase transition region with the aid of a full-wave retardation plate was conducted to observe the sign of birefringence for this mesophase. The result is confusing images that show disorganized birefringence with a negative value for the minute structures seen adjacent to the high density phase. The DNA concentration in this experiment is approximately 350 milligrams per milliliter, and the photomicrograph magnification is approximately 500x. Originally recorded on Fujichrome 64T transparency film using a Nikon Optiphot-Pol microscope with crossed polarized illumination, the image was digitized using a Nikon CoolScan transparency film scanner. Exposures were recorded about 3 f-steps under the recommended value given by an in-camera photomultiplier and were push-processed approximately 1.75 f-steps in the first E-6 developer.

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