The Precholesteric Mesophase

The Precholesteric Mesophase

Weak birefringence of the precholesteric liquid crystalline DNA mesophase is illustrated in the photomicrograph presented in this section. The large pitch and relative disorganization of this low-density anisotropic phase is evident in the image. The DNA concentration for this specimen is approximately 150 milligrams per millimeter, and the magnification is approximately 350x. The digital image presented above was originally recorded on Fujichrome 64T transparency film using a Nikon Optiphot-Pol microscope with crossed polarized illumination. Exposures were recorded about 2.5 f-steps under the recommended value given by an in-camera photomultiplier and were push-processed approximately 1.5 f-steps in the first E-6 developer.

© 1995-2013 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, software, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Thursday, Feb 26, 2004 at 12:44 PM
Access Count Since May 13, 2000: 28563