Observing Mitosis with Fluorescence Microscopy

Prometaphase

Presented in the digital fluorescence microscopy image above is a single rat kangaroo (PtK2) kidney cell in the early stages of prometaphase. The chromatin is stained with a blue fluorescent probe (DAPI), while the microtubule network (mitotic spindle) is stained green (Alexa Fluor 488) and cellular mitochondria are stained with a red dye (MitoTracker Red CMXRos). During prometaphase, the mitotic spindle microtubules are now free to enter the nuclear region, and formation of specialized protein complexes known as kinetochores begins on each centromere. These complexes become attached to a subset of the spindle microtubules, which are then termed kinetochore microtubules. Other microtubules in the spindle (not attached to centromeres) are termed polar microtubules, and these help form and maintain the spindle structure along with astral microtubules, which remain outside the spindle.


© 1995-2013 by Michael W. Davidson and The Florida State University. All Rights Reserved. No images, graphics, software, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
This website is maintained by our
Graphics & Web Programming Team
in collaboration with Optical Microscopy at the
National High Magnetic Field Laboratory.
Last modification: Monday, Dec 08, 2003 at 08:37 AM
Access Count Since December 8, 2003: 10502
Microscopes and digital imaging equipment provided by:
Visit the Olympus Microscopy Resource Center website. Visit the QImaging website.